Reactivity:
Rabbit
Application:
WB
Recommended Dilution:
ELISA 1:3000-1:8000
Western blotting 1:3000-1:8000
Dot blot 1:3000-1:8000
Source:
Goat
YH80561S Goat Anti-Rabbit Mouse IgG-HRP
Chemiluminescent detection systems have emerged as the all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives.
Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. Horseradish peroxidase (HRP) conjugated secondary antibodies are utilized in conjunction with specifc chemiluminescent substrates to generate the light signal. Horseradish peroxidase-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.