YH80023S His-Tag (10E2) mAb

Plasmid vectors for the expression of coding regions of eukaryotic genes in
bacterial, insect and mammalian hosts are in common usage; such expression
vectors are frequently used to encode hybrid fusion proteins consisting of a
eukaryotic target protein and a specialized region designed to aid in the
purification and visualization of the target protein. A system that has proven to
be very successful relies on the insertion of a six histidine (His6) sequence in
the N-terminus of the encoded protein, allowing for efficient coupling to Ni -
chelating resins and purification by single step affinity chromatography. This
polyhistidine sequence can then be removed by specific cleavage at sites
recognized by enzymes such as thrombin or enterokinase, permitting the
separation of the target protein from the polyhistidine tag. Visualization of
such fusion proteins can be achieved by utilizing antibodies generated against
specific peptide sequences downstream from the multiple cloning site.

20μl

1. Hui Wang, Yuchen Feng, Shuhui Zhang, Lulong Sun, Peng Yan, Yifeng Feng, and Zhengyang Zhao (2025). Phosphorylation of MdWRKY70L by MdMPK6/02G mediates reactive oxygen accumulation to regulate apple fruit senescence. Plant Biotechnology Journal, published on March 24, 2025.
2. Cong Sheng, Kaihuai Li, Bo Wang, Wenchan Chen, Baodian Guo, Lulu Qiao, Hongwei Zhao, Yancun Zhao, and Fengquan Liu (2025). OsPRMT5 methylates OsPAL1 to promote rice resistance, hindered by a Xanthomonas oryzae effector. Journal of Integrative Plant Biology, published on March 24, 2025.
3. Cheng Ke, Lin Duo, Ma Liqun, Lu Yao, Li Jinyan, Zhu Guoning, Lin Tao, Qu Guiqin, Zhu Benzhong, Fu Daqi, Luo Yunbo, and Zhu Hongliang (2025). A SlRBP1-SlFBA7/SlGPIMT module regulates fruit size in tomato. Horticulture Research, published on March 18, 2025.
4. Yansong Fu, Yunpeng Liu, Yu Chen, Jintao Xiao, Yuanming Xie, Youzhi Miao, Zhihui Xu, Nan Zhang, Weibing Xun, Wei Xuan, Qirong Shen, and Ruifu Zhang (2025). A rhizobacterium-secreted protein induces lateral root development through the IAA34-PUCHI pathway. Cell Reports, published on March 11, 2025.