Reactivity：
All
Application：
WB
Recommended Dilution：
Western blotting 1:5000 Immunofluorescence 1:1000 Immunoprecipitation 1:100 ELISA 1:2000
Immunogen：
This YH monoclonal antibody is produced by immunizing animals with GST.
Source：
Mouse
Specificity：
GST-Tag (12G8) Mouse mAb detects GST and GST fusion proteins.
YH80018S Anti-GST-Tag Antibody(HRPConjugated)
Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase
(GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.20μl
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- Dai Yao, Idorn Manja, Serrero Manutea C., Pan Xiaoyong, Thomsen Emil A., Narita Ryo, Maimaitili Muyesier, Qian Xiaoqing, Iversen Marie B., Reinert Line S., Flygaard Rasmus K., Chen Muwan, Ding Xiangning, Zhang Bao-cun, Carter-Timofte Madalina E., Lu Qing, Jiang Zhuofan, Zhong Yiye, Zhang Shuhui, Da Lintai, Zhu Jinwei, Denham Mark, Nissen Poul, Mogensen Trine H., Mikkelsen Jacob Giehm, Zhang Shen-Ying, Casanova Jean-Laurent, Cai Yujia, and Paludan Søren R. (2024). TMEFF1 is a neuron-specific restriction factor for herpes simplex virus. Nature, published on July 24, 2024.