YH80005S Anti- EGFP Rabbit Polyclonal Antibody

This polyclonal antibody was raised by immunizing rabbit with the entire EGFP molecule (recombinant protein).

Western Blot analysis (WB): 1,000 ~ 25,000 dilution

ELISA: 2,000 ~ 20,000 dilution

IP: 500 ~ 1,000 dilution

1. Protein sample run SDS-PAGE.
2. Bloting with semi-dry electrophoretic transfer system.
3. After transfering, rinse the transfer membrane with ddWater and let it dry.
4. Blocking transfer membrane with 5% skim milk, 0.2% sodium azide inTBS, incubate at 37℃ for at least 2 hrs or 4℃ O/N.
5. Wash the membrane with 0.05% Tween 20 in TBS (TBST).
6. Add primary Ab sol. and incubate at 37℃ for 1 hr. (Ab is diluted in 5% Goat serum/TBS)
7. Wash the membrane 3 times with TBST.
8. Add secondary Ab (Goat anti-rabbit IgG 3000X diluted in 5% Goat serum/TBS) and incubate at 37℃ for 1 hr.
9. Wash the membrane 3 times with TBST.
10. Wash the membrane with ddW.
11. Soak the membrane in BCIP/NBT substrate solution, after the color developed, wash with ddWater and let it dry.

Store this product at -20℃. The product is stable for one year from the date of shipment.

30ug/per tube

Con. 30mg/mL