YH80005S Anti- EGFP Rabbit Polyclonal Antibody

The Enhanced Green Fluorescent Protein (EGFP) is a 29kDa recombinant protein with Ex/Em = 488/507 nm. The green fluorescent protein was expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence. The EGFP protein is suitable as control reagents for expression studies.

This polyclonal antibody was raised by immunizing rabbit with the entire EGFP molecule (recombinant protein).

Western Blot analysis (WB): 1,000 ~ 25,000 dilution

ELISA: 2,000 ~ 20,000 dilution

IP: 500 ~ 1,000 dilution

1. Protein sample run SDS-PAGE.
2. Bloting with semi-dry electrophoretic transfer system.
3. After transfering, rinse the transfer membrane with ddWater and let it dry.
4. Blocking transfer membrane with 5% skim milk, 0.2% sodium azide inTBS, incubate at 37℃ for at least 2 hrs or 4℃ O/N.
5. Wash the membrane with 0.05% Tween 20 in TBS (TBST).
6. Add primary Ab sol. and incubate at 37℃ for 1 hr. (Ab is diluted in 5% Goat serum/TBS)
7. Wash the membrane 3 times with TBST.
8. Add secondary Ab (Goat anti-rabbit IgG 3000X diluted in 5% Goat serum/TBS) and incubate at 37℃ for 1 hr.
9. Wash the membrane 3 times with TBST.
10. Wash the membrane with ddW.
11. Soak the membrane in BCIP/NBT substrate solution, after the color developed, wash with ddWater and let it dry.

Store this product at -20℃. The product is stable for one year from the date of shipment.

30ug/per tube

Con. 30mg/mL