YH80004 Anti-6xHis Tag Rabbit Polyclonal Antibody
The 6x histidine recombinant protein tag is made up of 6 histidine residues attached to either the amino or carboxy terminus of the protein being expressed. The recombinant protein can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6x histidine in techniques such as western blotting and immunoprecipitation.
Rabbit polyclonal antibody raised against a 6x histidine peptide conjugated to OVA.
Western Blotting: 1:1,000 ~ 1:10,000
ELISA: 2,000 ~ 20,000 dilution
IP: 500 ~ 1,000 dilution
Liquid (containing 50% glycerol)
Store this product at -20℃. The product is stable for one year from the date of shipment.
100ug/per tube
Con. 100mg/mL