A ready-to-use kit for labeling antibodies with sulfo-Cyanine5 dye. This kit contains all necessary components (chemicals and consumables) to perform 10 labelings of antibodies (up to 100 ug antibody per each reaction). Reaction clean-up is achieved using enclosed gel filtration spin columns.
Precise quantification of the dye guarantees the reproducible outcome of the labeling and number of fluorophores per antibody molecule.
Purified labeled antibodies are available in PBS buffer after 40 min (10 min hands-on time). The kit tolerates common additives such as Tris, glycerol, and sodium azide.