SIMA (dichloro-diphenyl-fluorescein) is a dye with spectral properties similar to HEX but with a higher quantum yield.
SIMA azide is used to produce fluorescently labeled primers and hybridization probes for quantitative PCR. Oligonucleotides with a SIMA label can be easily generated via azide-alkyne cycloaddition between SIMA azide and alkyne-containing oligonucleotide.
SIMA azide, 6-isomer
Appearance: orange powder Molecular weight: 679.52 Molecular formula: C36H24Cl2N4O6 Solubility: good in DMSO, DMF Quality control: NMR 1H and HPLC-MS (95+%) Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.