ROX azide is an alkyne-reactive derivative of ROX (Rhodamine X, Rhodamine 101) dye. ROX is a red-emitting fluorophore possessing high brightness and fluorescence quantum yield. This reagent is a pure 5-isomer. It is used for labeling alkyne and cycloalkyne-containing biomolecules via copper-catalyzed and copper-free click chemistry reactions.
ROX azide, 5-isomer
Appearance: dark crimson powder / red solution Molecular weight: 616.71 CAS number: 2628213-67-0 Molecular formula: C36H36N6O4 Solubility: good in polar organic solvents (DMF, DMSO, alcohols), low in water Quality control: NMR 1H, HPLC-MS (95%) Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Excitation/absorption maximum, nm: 570 ε, L⋅mol−1⋅cm−1: 93000 Emission maximum, nm: 591 Fluorescence quantum yield: 1.0 CF260: 0.62 CF280: 0.49 Cat. # Quantity A1230 1 mg B1230 5 mg C1230 10 mg D1230 25 mg E1230 50 mg - Ferguson, T.E.G.; Reihill, J.A.; Martin, S.L.; Walker, B. Novel Inhibitors and Activity-Based Probes Targeting Trypsin-Like Serine Proteases. Frontiers in Chemistry, 2022, 10, 782608. doi: 10.3389/fchem.2022.782608
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