It is used intercalated Eva488 dye (EvaGreen® analog), that bound to double-stranded DNA and does not inhibit the reaction and exhibits a high fluorescence signal increase.
Used for PCR without fluorescent probes.
Eva488 Lyophilized qPCR kit reaction mix does not contain ROX reference dye thus it is compatible with any type real-time DNA amplifiers. The kit contains hot start (HS) polymerase which prevents non-specific amplification. Reaction mix volume 1 mL is good for 100 rxn of 20 µL.
Kit components:
- Component #1: Lyophilized HS Taq polymerase composition, 100 rxn
- Component #2: Polymerase reconstitution buffer, containing intercalating dye Eva488 a real-time PCR dye
Compatibility with equipment: is compatible with any type of thermocyclers.
Eva488 Lyophilized qPCR kit
Storage conditions: Transportation: at room temperature for up to 3 weeks. Store at -20 °С.