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Thiol-reactive, near-infrared dye Cyanine7.5 for labeling of protein SH groups, an analog of Cy7.5® maleimide.

Most proteins contain sulfhydryl groups which can be selectively labeled with maleimides. With this reagent, these proteins can be converted to near-infrared (NIR) fluorescent conjugates. After it, the conjugates can be used in non-invasive live organism imaging to study distribution of the protein in tissues and organs.

 

Lumiprobe 全新推出生命科學研究中最受歡迎的花青染料 —— Cyanine 系列。這些染料為 1970 年代廣泛應用於血管造影的經典花青染料吲哚菁綠(ICG)之改良版,具備更低的非特異性生物分子結合特性,並因其高消光係數及優異量子產率,展現出明亮且穩定的螢光表現。隨著 CMU 專利到期,這些高效能分子現已以更親民的價格,廣泛應用於各類生命科學領域。Cyanine 染料可用於標記多種目標,包括可溶性蛋白質、抗體、DNA 與寡核苷酸、胜肽(Peptides)及各類小分子。

Cyanine7.5 maleimide

  • Appearance: green powder
    Molecular weight: 807.46
    CAS number: 2270866-73-2; 2270866-72-1
(inner salt)
    Molecular formula: C51H55ClN4O3
    Solubility: soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
    Quality control: NMR 1H, HPLC-MS (95%)
    Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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