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Cyanine5 maleimide is a mono-reactive dye which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to give labeled conjugates.

Cyanine5 is an analog of Cy5®, a common fluorophore which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers.

For the labeling of antibodies and sensitive proteins we recommend to use the water soluble sulfo-Cyanine5 maleimide.

 

Lumiprobe 全新推出生命科學研究中最受歡迎的花青染料 —— Cyanine 系列。這些染料為 1970 年代廣泛應用於血管造影的經典花青染料吲哚菁綠(ICG)之改良版,具備更低的非特異性生物分子結合特性,並因其高消光係數及優異量子產率,展現出明亮且穩定的螢光表現。隨著 CMU 專利到期,這些高效能分子現已以更親民的價格,廣泛應用於各類生命科學領域。Cyanine 染料可用於標記多種目標,包括可溶性蛋白質、抗體、DNA 與寡核苷酸、胜肽(Peptides)及各類小分子。

Cyanine5 maleimide

  • Appearance: dark blue powder
    Molecular weight: 605.8
    CAS number: 1437872-46-2 (without anion)
    Molecular formula: C38H45N4O3
    IUPAC name: 3H-​Indolium, 2-​[5-​[1-​[6-​[[2-​(2,​5-​dihydro-​2,​5-​dioxo-​1H-​pyrrol-​1-​yl)​ethyl]​amino]​-​6-​oxohexyl]​-​1,​3-​dihydro-​3,​3-​dimethyl-​2H-​indol-​2-​ylidene]​-​1,​3-​pentadien-​1-​yl]​-​1,​3,​3-​trimethyl-
    Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), practically insoluble in water (31 uM, 23 mg/L)
    Quality control: NMR 1H, HPLC-MS (95%)
    Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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