top of page

Cyanine5.5 is a fluorophore with an emission in the far-red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.

This phosphoramidite can be used for the synthesis of 5'-labeled oligonucleotides by direct labeling in an oligonucleotide synthesizer.

The structure of the phosphoramidite functional group that is attached to a secondary carbon atom provides extra stability against Arbuzov rearrangement. This helps maintain coupling performance over a longer storage time in oligonucleotide synthesizers, compared to phosphoramidites derived from primary alcohols.

 

Lumiprobe 全新推出生命科學研究中最受歡迎的花青染料 —— Cyanine 系列。這些染料為 1970 年代廣泛應用於血管造影的經典花青染料吲哚菁綠(ICG)之改良版,具備更低的非特異性生物分子結合特性,並因其高消光係數及優異量子產率,展現出明亮且穩定的螢光表現。隨著 CMU 專利到期,這些高效能分子現已以更親民的價格,廣泛應用於各類生命科學領域。Cyanine 染料可用於標記多種目標,包括可溶性蛋白質、抗體、DNA 與寡核苷酸、胜肽(Peptides)及各類小分子。

Cyanine5.5 phosphoramidite

  • Appearance: dark colored solid
    Molecular weight: 916.61
    Molecular formula: C55H71N5ClO3P
    Quality control: NMR 1H, 31P, HPLC-MS (85%)
    Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
bottom of page