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Cyanine3B DBCO

Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.

Cyanine3B is a yellow-emitting cyanine dye that is an improved version of the Cyanine3 fluorophore with significantly higher fluorescence quantum yield and photostability. Due to the fixed conformation, Cyanine3B has the highest emission quantum yield compared to other dyes of this wavelength.

This is a sulfonated dye and can be used for efficient labeling in water media.

 

Lumiprobe 全新推出生命科學研究中最受歡迎的花青染料 —— Cyanine 系列。這些染料為 1970 年代廣泛應用於血管造影的經典花青染料吲哚菁綠(ICG)之改良版,具備更低的非特異性生物分子結合特性,並因其高消光係數及優異量子產率,展現出明亮且穩定的螢光表現。隨著 CMU 專利到期,這些高效能分子現已以更親民的價格,廣泛應用於各類生命科學領域。Cyanine 染料可用於標記多種目標,包括可溶性蛋白質、抗體、DNA 與寡核苷酸、胜肽(Peptides)及各類小分子。

Cyanine3B DBCO

  • Appearance: dark red powder
    Molecular weight: 861.08
    Molecular formula: C52H52N4O6S
    Solubility: soluble in methanol, ethanol, DMF, DMSO
    Quality control: NMR 1H and HPLC-MS (90+%)
    Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
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