Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).
Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.
Labeling with Cyanine3 maleimide is selective, and efficient.
We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.
 
Lumiprobe 全新推出生命科學研究中最受歡迎的花青染料 —— Cyanine 系列。這些染料為 1970 年代廣泛應用於血管造影的經典花青染料吲哚菁綠（ICG）之改良版，具備更低的非特異性生物分子結合特性，並因其高消光係數及優異量子產率，展現出明亮且穩定的螢光表現。隨著 CMU 專利到期，這些高效能分子現已以更親民的價格，廣泛應用於各類生命科學領域。Cyanine 染料可用於標記多種目標，包括可溶性蛋白質、抗體、DNA 與寡核苷酸、胜肽（Peptides）及各類小分子。