Cyanine3.5 is a bright and photostable fluorophore, which is similar in its spectral characteristics to Cy3.5™ dye. Cyanine3.5 DBCO fluorescence maximum is at 604 nm and in the orange-red spectrum range.
Cyanine3.5 fluorescence is pH insensitive in the range from 4 to 10 and can be detected in the spectrum range with a low level of autofluorescence of biological samples.
Cyanine3.5 DBCO (dibenzocyclooctyne) easily forms conjugates with azide derivatives of biomolecules in copper-free Click Chemistry. The process runs without catalysts or increased temperature, resulting in stable triazoles.
Conjugates of biomolecules with Cyanine3.5 can be used for various microscopy assays, including FRET-microscopy, and in proteomics.
 
Lumiprobe推出了生命科學研究中最受歡迎的花青染料Cyanine。這些染料是自 1970 年代以來用於血管造影的花青染料吲哚菁綠 (ICG) 的改進型，並發現這些染料與生物分子的非特異性結合較低，且由於其巨大的消光係數和良好的量子產率而具有明亮的螢光。這些分子在 CMU 專利到期後，現正以親善的價格廣泛地進入生命科學應用等領域。花青染料Cyanine可用於標記許多類別的目標，包括：可溶性蛋白質、抗體、DNA 和寡核苷酸、Peptides、許多小分子。