Non-nucleosidic modifier reagent for the synthesis of biotinylated oligos based on a hydroxyprolinol core. This phosphoramidite contains a dimethoxytrityl protective group for the purification of the resulting oligos on C18 cartridges, and reverse phase HPLC. This modifier reagent can be used to introduce biotin onto 5'-position, 3'-position (using universal CPG), and internal oligo sites.
This amidite does not require special condensation and deprotection conditions.
Biotin phosphoramidite (hydroxyprolinol)
Appearance: white powder Molecular weight: 959.18 Molecular formula: C51H71N6O8PS Solubility: good in MeCN, DCM, THF Quality control: NMR 1H, 31P, HPLC-MS (95%), functional testing in oligo synthesis Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.