BDP 558/568 has a high molar extinction coefficient and high quantum yield; this is a bright fluorophore and an alternative to BDP 558/568 and Cy3™ because of similar spectral properties. BDP 558/568 can be used in two-photon microscopy; it has a long excited-state lifetime, so it can be used in fluorescence polarization assay.
BDP 558/568 tetrazine is a convenient reagent for producing fluorescent conjugates of proteins, nucleic acids, and other biomolecules by tetrazine-trans-cyclooctene (TCO) ligation. This cycloaddition reaction runs relatively rapidly without metal catalysts.
BDP® 558/568 tetrazine
Appearance: brown powder Molecular weight: 529.37 Molecular formula: C26H22N7BF2OS IUPAC name: 3-(5,5-difluoro-7-(thiophen-2-yl)-5H-5l4,6l4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)-N-(4-(6-methyl-1,2,4,5-tetrazin-3-yl)benzyl)propanamide Solubility: very soluble in DMSO and DMF Quality control: NMR 1H, HPLC-MS (95%) Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.