C-Terminal
AS20 4510-1ml | Clonality: Monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana
AS20 4510-1ml Anti-ARP8 | Actin-related protein 8, (clone 12F5,D4,C3)
Sub class: IgG1 Immunogen: MAP- conjugated peptide of Arabidopsis thaliana ARP8, C-terminal UniProt: Q9FKT0-1, TAIR: At5g56180 Host: Mouse Clonality: Monoclonal Purity: Cell culture supernatant Format: Liquid Quantity: 1 ml Storage: Store at -20°C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Immunocytochemistry (ICC), Immunoflourescence (IF), Western blot (WB) Recommended dilution: assay dependent Expected | apparent MW: 52 kDa Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Species of your interest not listed? Contact us Not reactive in: No confirmed exceptions from predicted reactivity are currently known Antibody is recognizing following epitope, amino acids 447-471: SNLSIFPGPWCITRKQFRRKSRLMW
This antibody is recognizing the full-length 52 kDa recombinant ARP8 protein expressed in Escherichia coli as well as endogenous ARP8 of identical molecular weight in Arabidopsis thaliana extracts from different tissues: all vegetative and reproductive organs examined including seedlings, roots and siliques, with higher concentrations of the protein detected in developing flower buds and flowers within the inflorescence.Actin-related proteins (ARPs) are found in the nuclei of all eukaryotic cells, but their functions are generally understood only in the context of their presence in various yeast and animal chromatin-modifying complexes. Arabidopsis ARP8 shows 30 and 29% amino acid identity to yeast actin and Arabidopsis ACT2 in the regions of alignment, respectively. Because it is not closely related to yeast or human ARP8 and shows similar weak homology to yeast ARP8 and ARP9, the Arabidopsis ARP8 is considered a plant-specific orphan ARP.
- Kandasamy et al. (2008). ACTIN-RELATED PROTEIN8 encodes an F-box protein localized to the nucleolus in Arabidopsis. Plant Cell Physiol. 49(5):858-63. doi: 10.1093/pcp/pcn053.