AS19 4350 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS19 4350 Anti-ACC synthase 8 | 1-aminocyclopropane-1-carboxylate synthase 8
Immunogen: Recombinant Arabidopsis thaliana 1-aminocyclopropane-1-carboxylate synthase 8 protein, amino acids: 1-469, Uniprot: Q9T065, TAIR: AT4G37770 Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein G purified. Format: Liquid Quantity: 50 µl Storage: Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 53 kDa Confirmed reactivity: Arabidopsis thaliana Not reactive in: No confirmed exceptions from predicted reactivity are currently known 80 and 40 µg of recombinant ACS8 protein were separated on 8 % SDS-PAGE and blotted 1h to PVDF. Blot was blocked with 5 % milk for 2h/RT in PBS-T with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h/RT with agitation in PBS-T or ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4x in PBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent according to manufacture's instructions.
Additional information: Preservative: 0,03% Proclin 300, Preparation contains: 50% Glycerol, 10 mM PBS, pH 7,4 Additional information (application): Reactivity of this antibody on endogenous protein remains to be determined ACC synthase 8 belongs to a group fo enzymes which catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene. ACS8 is auxin inducible.
Alternative names: 1-aminocyclopropane-1-carboxylate synthase 8, ACC synthase 8, S-adenosyl-L-methionine methylthioadenosine-lyase 8.