AS19 4280 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS19 4280 Anti-ATG16 | Autophagy-related protein 16
Immunogen: N-terminal part of of ATG16 of Arabidopsis thaliana UniProt: Q6NNP0, TAIR: At5g50230 Host: Rabbit
Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl, of sterile water Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 56.4 | >70 kDa Confirmed reactivity: Arabidopsis thaliana Not reactive in: No confirmed exceptions from predicted reactivity are currently known Reactant: Aponogeton madagascariensis
Application: Western Blotting
Pudmed ID: 36795694
Journal: PLoS One
Figure Number: 2C
Published Date: 2023-02-17
First Author: Rowarth, N. M.
Impact Factor: 3.582
Detection of lace plant Atg16 in lace plant leaves.RNA and protein were extracted and probed for Atg16 from imperforate (I), pre-perforation (P), window (W), or mature (M) lace plant leaf stages (A-D) or only window and mature leaves from plants treated with either 5 μM rapamycin (Rap), 1 μM wortmannin (Wrt), and 1 μM concanamycin A (ConA) compared to DMSO control (E-H). (A and E) The mean levels of Atg16 mRNA in different lace plant leaf stages were determined by qRT-PCR and normalized to lace plant α-tubulin levels. Protein extracts and a molecular protein standard (L) were resolved by SDS–polyacrylamide gels and blotted to nitrocellulose membranes. (B and F) Membranes and protein lanes were stained with Ponceau-S to serve as loading control before subsequent detection of the presence or absence of ~56 kDa sized Atg16 protein bands with anti-Atg16 antibody (C and G). (D and H) Immunoreactive protein bands were quantitated, and the ratio of Atg16 band intensity to the Ponceau lane signal was averaged. The experiments were performed in triplicate. Means not sharing any letter are significantly different. One-way ANOVA (A, D and H), or Two-way ANOVA, Tukey test (E, P < 0.05; n = 3). Error bars represent the SE.
Reactant: Aponogeton madagascariensis
Application: Western Blotting
Pudmed ID: 36795694
Journal: PLoS One
Figure Number: 2G
Published Date: 2023-02-17
First Author: Rowarth, N. M.
Impact Factor: 3.582
Detection of lace plant Atg16 in lace plant leaves.RNA and protein were extracted and probed for Atg16 from imperforate (I), pre-perforation (P), window (W), or mature (M) lace plant leaf stages (A-D) or only window and mature leaves from plants treated with either 5 μM rapamycin (Rap), 1 μM wortmannin (Wrt), and 1 μM concanamycin A (ConA) compared to DMSO control (E-H). (A and E) The mean levels of Atg16 mRNA in different lace plant leaf stages were determined by qRT-PCR and normalized to lace plant α-tubulin levels. Protein extracts and a molecular protein standard (L) were resolved by SDS–polyacrylamide gels and blotted to nitrocellulose membranes. (B and F) Membranes and protein lanes were stained with Ponceau-S to serve as loading control before subsequent detection of the presence or absence of ~56 kDa sized Atg16 protein bands with anti-Atg16 antibody (C and G). (D and H) Immunoreactive protein bands were quantitated, and the ratio of Atg16 band intensity to the Ponceau lane signal was averaged. The experiments were performed in triplicate. Means not sharing any letter are significantly different. One-way ANOVA (A, D and H), or Two-way ANOVA, Tukey test (E, P < 0.05; n = 3). Error bars represent the SE.
ATG16 (Autophagy-related protein 16) may play a role in autophagy process.