AS19 4278  | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

AS19 4278 Anti-ATG12b | Autophagy-related protein 12b

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Immunogen:	Recombinant, full-length ATG12b ofArabidopsis thaliana protein sequence UniProt: Q9LVK3-1, TAIR: At3g13970
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
Reconstitution:	For reconstitution add 50 µl, of sterile water
Storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 3000 (WB)
Expected \| apparent MW:	10.4 \| 12 and 50 kDa in wilde type plants and 12 kDa in some autophagy-deficient mutants

5 µg (1,3) and 2.5 µg (2,5) of lysate from E.coli cells expressing CCaP1 protein/lane were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-CCaP1 antibodies (AS09 483, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
Chung et al. (2010). ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis require ATG12 expressed from the differentially controlled ATG12A AND ATG12B loci. Plant J. 2010 May;62(3):483-93. doi: 10.1111/j.1365-313X.2010.04166.x

Confirmed reactivity:	Arabidopsis thaliana
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

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