AS19 4277 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS19 4277 Anti-ATG7 | Autophagy-related protein 7
Immunogen: Recombinant ATG7 of Arabidopsis thaliana, UniProt: Q94CD5-1, TAIR: At5g45900, overexpressed in E.coli, collected in inclusion bodies, which were solubilized before immunization Host: Rabbit
Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl, of sterile water Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years); once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 3000 (WB) Expected | apparent MW: 76.5 | 75 kDa Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Brassica rapa, Capsella rubella, Citrus clementina, Noccaea caerulescens
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known Protein name was changed from APG7 to ATG7 after Doelling paper was published.
ATG7 (Autophagy-related protein 7) is an E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Acts in the senescence process, degradation of damaged peroxisomes and non-selective degradation of chlorophylls and photosynthetic proteins during stress-induced leaf yellowing.
Alternative names: Ubiquitin-like modifier-activating enzyme atg7, ATG12-activating enzyme E1 atg7, AtAPG7, Protein PEROXISOME UNUSUAL POSITIONING 4.- Doelling et al. (2002). The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana. J Biol Chem. 2002 Sep 6;277(36):33105-14.