AS19 4276 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS19 4276 Anti-ATG5 | Autophagy protein 5
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Immunogen: Full-length, recombinant ATG5 of Arabidopsis thaliana protein sequence UniProt: Q9FFI2-1, TAIR: At5g17290, overexpressed in E.coli Host: Rabbit
Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl, of sterile water Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 3000 (WB) Expected | apparent MW: 38.5 | 50 kDa (in wilde type plants) and ca. 38 kDa in autophagy defective mutants Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Solanum lycopersicum, Solanum tuberosum Not reactive in: No confirmed exceptions from predicted reactivity are currently known In wild-type plants almost all ATG5 is conjugated to ATG12.
This item can be sold containing ProClinATG5 (Autophagy protein 5) is a protein required for autophagy. Involved in a negative feedback loop that modulates NPR1-dependent salicylic acid (SA) signaling and limits senescence and immunity-related programmed cell death (PCD) in plants and a complete proteolysis of chloroplast stroma proteins in senescent leaves, in the degradation of damaged peroxisomes.
Alternative names: Protein autophagy 5, AtAPG5.- Thompson et al. (2005). Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways. Plant Physiol. 2005 Aug;138(4):2097-110.