AS19 4274 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS19 4274 Anti-ATG1a | Serine/threonine-protein kinase ATG1a
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Immunogen: Full-length, N-terminally HIS-tagged ATG1a of Arabidopsis thaliana protein sequence UniProt: Q94C95, TAIR: At3g61960, overexpressed in E.coli and injected as a gel piece Host: Rabbit
Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl, of sterile water Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 69.6 | 70 kDa Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Noccaea caerulescens
Species of your interest not listed? Contact usNot reactive in: Oryza sativa Antibody may also recognise ATG1b and ATG1c; does not recognise ATG1t, Suttangkakul et al. (2011).
ATG1a (Serine/threonine-protein kinase ATG1a) is a serine/threonine protein kinase involved in autophagy in a nutritional condition-dependent manner. The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Becomes a target of autophagy under nutrient starvation and connects autophagy to plant nutritional status.
Alternative names: Autophagy-related protein 1a, AtAPG1a.- Suttangkakul et al. (2011). The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis. Plant Cell. 2011 Oct;23(10):3761-79. doi: 10.1105/tpc.111.090993.