AS16 3141 Anti-ACT | Actin (monoclonal, clone mAbGPa 10-B3)

Immunogen:	Full-length recombinant Arabidopsis thaliana Actin-8 (ACT8)  expressed in E.coli, Uniprot: Q96293
Sub class:	IgG2b
Host:	Mouse
Clonality:	Monoclonal
Purity:	Immunoglobulin IgG2b, Protein G purified in 0.1 M Sodium Phosphate, pH 7.4. Contains 0.15 M NaCl, 0.05% (w/v) sodium azide.
Format:	Liquid
Quantity:	100 µg
Storage:	Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution:	1-2 µg/ml (WB)
Expected | apparent MW:	45 | 45 kDa

Confirmed reactivity:	Arabidopsis thaliana, Dictyostelium discoideum
Predicted reactivity:	Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Camelina sativa, Camellia lipoensis, Cucumis sativus, Dendrocalamus latiflorus, Dionaea muscipula, Gossypium sp., Eucalyptus grandis, Euphorbia lathyris, Gynura bicolor, Ficus microcarpa, Haloxylon ammodendron, Helianthus annuus, Hevea brasiliensis, Lilium regale, Medicago sativa, Mimosa pudica, Nitraria sibirica, Oryza sativa subsp. japonica, Oryza sativa subsp. indica, Oxytropis ochrocephala, Paeonia lactiflora, Panax notoginseng, Populus trichocarpa, Prunus avium, Ricinus communis, Striga asiatica, Theobroma cacao, Torenia fournieri, Trifolium pRatense, Ziziphus jujuba Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known.

This antibody is purified on a protein G column. It recognizes Arabidopsis actins ACT1, 2, 3, 4, 7, 8, 11, 12 and Dictyostelium actin

Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

1. Nuamzanei et al. (2024). Impact of polyvinyl chloride (PVC) microplastic on growth, photosynthesis and nutrient uptake of Solanum lycopersicum L. (Tomato). Environ Pollut. 2024 Apr 16:123994. doi: 10.1016/j.envpol.2024.123994.  
2. Sultan et al. (2017). The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria. Plant Cell. 2016 Nov;28(11):2805-2829.
3. Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281