AS16 3140 Anti-ACT 2,8,11 | Actin 2, 8, 11 (clone mAb13a)

Sub class:	IgG1
Immunogen:	Full-length recombinant Arabidopsis thaliana Actin-2 (ACT2) expressed in E.coli, Uniprot: Q96292
Host:	Mouse
Clonality:	Monoclonal
Purity:	IgG1 immunoglobulin purified on Protein G in 0.1 M Sodium Phosphate, pH 7.4, 0.15 M NaCl, 0.05% (w/v) sodium azide.
Format:	Liquid
Quantity:	100 µg
Storage:	Store at -20°C,Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution:	1-2 µg/ml (WB)
Expected | apparent MW:	45 | 45 kDa

Confirmed reactivity:	Arabidopsis thaliana, Nicotiana tabacum!!AIR8!!Arabidopsis thaliana, Nicotiana tabacum
Predicted reactivity:	Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Cucumis sativus, Dionea muscipula, Euphorbia lathyris, Ficus microcarpa, Gentlisea aurea, Gossypium sp., Hevea brasiliensis, Medicago sativa, Medicago truncatula, Oryza sativa subsp. japonica, Oxytropis ochrocephala, Panax notoginseng, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Striga asiatica, Theobrom cacao, Torenia fournieri, Trifolium pratense, Vaccinium ashei, Ziziphus jujuba Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Additional information:	This antibody is a total IgG fraction, purified on a protein G column. It recognizes actin isoforms ACT2, 8, and 11.
Additional information (application):	Exact working dilution needs to be determined by end user

Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

1. Kandasamy, M.K., et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell May;24(5):2041-57. doi: 10.1105/tpc.111.095281.
2. Kandasamy, M.K., et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54.
3. Kandasamy, M.K., et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-9.