AS16 3139 | Clonality: monoclonal | Host: Mouse | Reactivity: Arabidopsis thaliana
AS16 3139 Anti-ACT 1,3,4,12 | Actin 1, 3, 4, 12 (clone mAB45a (5-15 H7C5))
Immunogen: Full-length recombinant Arabidopsis thaliana Actin-1 (ACT1) expressed in E.coli, Uniprot: P0CJ46 Sub class: IgG1 Host: Mouse Clonality: Monoclonal Purity: IgG1 immunoglobulin purified on Protein G in 0.1 M Sodium Phosphate, pH 7.4, 0.15 M NaCl, 0.05% (w/v) sodium azide. Format: Liquid Quantity: 100 µg Storage: Store at -20°C,Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1-2 µg/ml (WB) Expected | apparent MW: 45 | 45 kDa
Confirmed reactivity: Arabidopsis thaliana!!AIR8!!Arabidopsis thaliana Predicted reactivity: Clonorchis sinensis, Cucurbita maxima, Cucumis sativus, Genlisea aurea, Glycine soja, Gossypium arboreum, Gossypium hirsutum, Litsea cubeba, Medicago truncatula, Narcissus tazetta var. chinensis, Phaseolus vulgaris, Platycodon grandiflorus, Paulownia tomentosa, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Ricinus communis, Solanum tuberosum, Striga asiatica, Theobroma cacao
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known Additional information: This antibody is a total IgG fraction purified on a protein G column, It recognizes the epitope Asn79 of actins ACT1, 3, 4, 12 Additional information (application): Exact working dilution needs to be determined by end user Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
- Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281
- Kandasamy, M.K. et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54
- Kandasamy, M.K. et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-91.