AS14 2774 Anti-ACC synthase 7 | 1-aminocyclopropane-1-carboxylate synthase 7

Immunogen:	KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ACC synthase 7, UniProt: Q9STR4,TAIR: AT4G26200
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	100 µg
Reconstitution:	For reconstitution add 100 µl of sterile water
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 500 (WB)
Expected | apparent MW:	50.6 kDa

Confirmed reactivity:	Arabidopsis thaliana
Predicted reactivity:	Arabidopsis thaliana
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

10 ug of total protein extract from Nicotiana benthamiana leaves expressed ACS7­His­GST (1), 5 ug of purified ACS7­His­GST from N. benthamiana ACS7­His­GFP fusion protein transiently expressed in N. benthamiana leaves was purified on Ni ‐ NTA resin(2) and (3). Samples were separated on 10% SDS­PAGE and transfered to PVDP membrane by semi­dry blotting (1h). Blot was blocked with 3% skim milk in PBS­T (0,01% Tween 20) for 1h in RT with agitation. Then blot was incubated with primary antibody anti ACS7 at dilution of 1:500 in PBS­T for 1h in RT with agitation. After washes (3 times for 5 min. in PBS­T) blot was incubated with secondary antibody (anti­rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) at dilution 1:25000 for 1h in RT. The blot was washed 5 times for 5 min. with PBS­T and developed for 2 min with ECL­Plus according to the manufacturer's instructions. Exposure time was 3 min.
 

Courtesy of Dr. Agata Cieśla, Ludwików's lab, UAM, Poland

ACS7 protein levels are too low to allow detection in endogenous cell extract

1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene. (EC:4.4.1.14). Synonymes: S-adenosyl-L-methionine methylthioadenosine-lyase 7.