AS12 2113 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS12 2113 Anti-AHK2 | Histidine kinase 2
Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AHK2 protein sequence, TAIR: AT5G35750, UniProt: Q9C5U2
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 131 kDa
Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Nicotiana benthamiana, Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known Reactivity of AHB2 antibodies to recombinant AHB1 and AHB2
ng of recombinant Arabidopsis thaliana AHB1 and AHB2 are indicated on a blot. The SDS gels are 15% acrylamide (28:1 acrylamide:bis-acrylamide). Transfer to PVDF occurred at 100 mAmp per gel for 90 minutes. Blocking solution was "blotto" (2.5 % (w/v) non-fat dry milk dissolved in TBST (TBS + 0.05% Tween-20)). Blocking occurred for 15 minutes. Blots were agitated at room temperature with 1:1000 anti-AHB2 primary antibody for 2 hours. Blot was incubated in secondary antibody at a concentration of 1:5000. Exposure time was consistently between 30 and 60 seconds.
Courtesy of: Damian Guerra; Laboratory of Elizabeth Vierling; University of Massachusetts, Amherst; Amherst, USA
AHB2 expression in stage 1-15 flowers
Indicated protein amounts of Arabidopsis thaliana extracts are indicated on a blot. The SDS gels are 15% acrylamide (28:1 acrylamide:bis-acrylamide). Transfer to PVDF occurred at 100 mAmp per gel for 90 minutes. Blocking solution was "blotto" (2.5 % (w/v) non-fat dry milk dissolved in TBST (TBS + 0.05% Tween-20)). Blocking occurred for 15 minutes. Blots were agitated at room temperature with 1:1000 anti-AHB2 primary antibody for 2 hours. Blot was incubated in secondary antibody at a concentration of 1:5000. Exposure time was consistently between 30 and 60 seconds.
Courtesy of: Damian Guerra; Laboratory of Elizabeth Vierling; University of Massachusetts, Amherst; Amherst, USAAHK2 (Histidine kinase 2) is a cytokinin receptor, multi-pass membrane protein which transmits the stress signal to a downstream MAPK cascade.
Alternative name: Authentic HIS-Kinase 2.