AS12 2113 Anti-AHK2 | Histidine kinase 2

Reactivity of AHB2 antibodies to recombinant AHB1 and AHB2
ng of recombinant Arabidopsis thaliana AHB1 and AHB2 are indicated on a blot. The SDS gels are 15% acrylamide (28:1 acrylamide:bis-acrylamide). Transfer to PVDF occurred at 100 mAmp per gel for 90 minutes. Blocking solution was "blotto" (2.5 % (w/v) non-fat dry milk dissolved in TBST (TBS + 0.05% Tween-20)). Blocking occurred for 15 minutes. Blots were agitated at room temperature with 1:1000 anti-AHB2 primary antibody for 2 hours. Blot was incubated in secondary antibody at a concentration of 1:5000. Exposure time was consistently between 30 and 60 seconds.

Courtesy of: Damian Guerra; Laboratory of Elizabeth Vierling; University of Massachusetts, Amherst; Amherst, USA  
 

AHB2 expression in stage 1-15 flowers
Indicated protein amounts of Arabidopsis thaliana extracts are indicated on a blot. The SDS gels are 15% acrylamide (28:1 acrylamide:bis-acrylamide). Transfer to PVDF occurred at 100 mAmp per gel for 90 minutes. Blocking solution was "blotto" (2.5 % (w/v) non-fat dry milk dissolved in TBST (TBS + 0.05% Tween-20)). Blocking occurred for 15 minutes. Blots were agitated at room temperature with 1:1000 anti-AHB2 primary antibody for 2 hours. Blot was incubated in secondary antibody at a concentration of 1:5000. Exposure time was consistently between 30 and 60 seconds.

Courtesy of: Damian Guerra; Laboratory of Elizabeth Vierling; University of Massachusetts, Amherst; Amherst, USA  

AHK2 (Histidine kinase 2) is a cytokinin receptor, multi-pass membrane protein which transmits the stress signal to a downstream MAPK cascade.

Alternative name: Authentic HIS-Kinase 2.