AS09 483 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS09 483 Anti-AtCCaP1 | vacuolar calcium-binding protein-related
Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCaP1m UniProt: Q9SXE9, TAIR: At1g62480
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 100 µl Storage: Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1 : 8000 (ELISA), 1 : 1000 (WB) Expected | apparent MW: 16.6 kDa Confirmed reactivity: Arabidopsis thaliana Predicted reactivity: Arabidopsis thaliana Not reactive in: No confirmed exceptions from predicted reactivity are currently known 5 µg (1,3) and 2.5 µg (2,5) of lysate from E.coli cells expressing CCaP1 protein/lane were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-CCaP1 antibodies (AS09 483, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
Additional information: 0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.
Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
Additional information (application): Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
CCaP1 acts as a mediator in response to continuous dark or gibberellic acid. Alternative name: At1g62480/T3P18_4, T3P18.4
- Ide et al. (2007). Transcriptional Induction of Two Genes for CCaPs, Novel Cytosolic Proteins, in Arabidopsis thaliana in the Dark. Plant Cell Physiol. 1:54-65.
Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extractsAgrisera Educational Posters Collection
Courtesy of Dr. Masayoshi Maeshima, Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences Nagoya University Nagoya, Japan