AS09 446 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Eucalyptus globulus, Pinus radiata
AS09 446 Anti-ABA | Abscisic acid (C1) (for immunolocalization)
Immunogen: BSA-conjugated abscisic acid (C1) via C1 carboxyl group
Host: Rabbit Clonality: Polyclonal Purity: Total IgG. Protein G purified in PBS with 50 % glycerol. Format: Liquid Quantity: 200 µg Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunofluorescence (IF), Immunolocalization (IL) Recommended dilution: The optimal working dilution should be determined by the investigator Confirmed reactivity: Abscisic acid (C1) in Arabidopsis thaliana, Eucalyptus globulus, Petunia hybrida L., Pinus radiata, Populus trichocarpa Predicted reactivity: Abscisic acid (C1) Not reactive in: No confirmed exceptions from predicted reactivity are currently known Reactant: Populus trichocarpa (Black cottonwood)
Application: Immunohistochemistry-immunofluorescence
Pudmed ID: 32192046
Journal: Int J Mol Sci
Figure Number: 7A,B,C,D,E,F
Published Date: 2020-03-17
First Author: Wojciechowska, N., Wilmowicz, E., et al.
Impact Factor: 5.542
Open Publication
Immunolocalization of ABA (green fluorescence) during senescence process of fine, absorptive roots. (A,B) Root control (RC); (C,D) The first stage of root senescence (RS1); (E,F) The second stage of root senescence (RS2). Autofluorescence (red) of the cell wall was registered in order to visualize the cell/organ shape. Abbreviations: R, rhizodermis; C, parenchyma cortex cells; Ct, cytoplasm; Ph, phloem, X, xylem. Scale bars = 50 µm.
Reactant: Populus trichocarpa (Black cottonwood)
Application: Immunohistochemistry-immunofluorescence
Pudmed ID: 32192046
Journal: Int J Mol Sci
Figure Number: 8A,B,C,D,E,F,G,H,I
Published Date: 2020-03-17
First Author: Wojciechowska, N., Wilmowicz, E., et al.
Impact Factor: 5.542
Immunodetection of ABA (green fluorescence) and red autofluorescence of chlorophyll during leaf senescence. (A–C) Leaves control (LC); (D–F) The first stage of leaf senescence (LS1); (G–I), The second stage of leaf senescence (LS2). Abbreviations: VB, vascular bundle; PM, palisade mesophyll; SM, spongy mesophyll; E – epidermis. Scale bars = 50 µm.
Additional information: ABA | Abscisic acid (C1) (for immunolocalization) Additional information (application): The antibody will recognize either the ABA conjugated to glucose ester (ABA-GE) or the ABA precursor: abscisic acid aldehyde, ABA aldehyde is however not usually present in plant tissue similarly to ABA alcohol which is also reactive, The antibodies will predominantly recognize only ABA and its glucosylester Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.
- Wojciechowska et al. (2020). Abscisic Acid and Jasmonate Metabolisms Are Jointly Regulated During Senescence in Roots and Leaves of Populus trichocarpa. Int J Mol Sci , 21 (6)
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