Phenylethynylpyrene (PEP) fluorophore is a polyaromatic hydrocarbon label with high sensitivity to microenvironment. Similarly to pyrene, PEP dye readily forms excimers. However, AF 384 (PEP) fluorescence is more red-shifted
PEP can be used as microenvironment probe, and as a label for assays based on excimer formation.
This reagent contains triethyleneglycol linker to facilitate dissolution of non-polar PEP dye in organo-aqueous labeling reaction mixtures. With this azide, and click chemistry, it is easy to turn any molecule bearing alkyne into PEP-labeled probe.
AF 384 (PEP) azide
Appearance: off white / yellowish solid Molecular weight: 544.64 CAS number: 1807521-02-3 Molecular formula: C34H32N4O3 Solubility: soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile Quality control: NMR 1H (95%) Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.