Why Choose Gene Synthesis?
Gene synthesis has emerged as the most cost-effective, time-saving, and resource-efficient method, allowing you to easily obtain virtually any DNA sequence you need. Traditional methods like PCR, mutagenesis, and subcloning are often time-consuming and frequently limited by sample quality and chance. With gene synthesis, you will acquire stable and high-quality sequences, and you can also implement optimized designs to effectively enhance protein expression efficiency. More importantly, gene synthesis eliminates the limitations of natural samples and frees you from concerns about source material, making it a crucial tool for resolving research interruptions and accelerating experimental progress.
Beyond saving time and ensuring accuracy, you can also freely customize restriction enzyme sites and select expression vectors and host systems according to your experimental needs. Coupled with Yao-Hong's complimentary sequence optimization analysis, you can further fine-tune your gene design to better suit your chosen expression platform. Whether it involves multiple point mutations, sequence reconstruction, or special fragments like high GC content and highly repetitive sequences, all can be easily achieved.
Precision and Accuracy
Time-Saving
Cost-Effective
Comprehensive Customization
Double verification, mutation-free
Deliver results within weeks
Lower cost than in-house experimentation
Vectors and restriction sites customizable at will
Yao-Hong's Service Advantages
Yao-Hong Biotech combines extensive gene design experience with advanced software analysis technology to provide you with rapid and highly efficient gene synthesis services:
-
Codon optimization analysis considering mRNA structure and host preference to enhance protein expression levels.
-
Construction of challenging and complex sequences, including high GC content, repetitive sequences, and hairpin structures.
-
Support for various vectors and restriction enzyme site selections, offering high flexibility, including the option for seamless homologous recombination.
-
Free subcloning vectors provided, with pUC57 as the default, and over 150 expression vectors available for selection.
-
High efficiency, competitive pricing, and turnaround time, with special offers for fragment lengths under 3000 bp.
-
Complimentary codon optimization service to help increase recombinant protein yield and activity.
-
100% sequence accuracy guarantee, verified by dual QC through Sanger sequencing and restriction enzyme digestion.
-
Single synthesis capacity up to 50,000 bp, suitable for large fragment design and construction.
Complimentary Codon Optimization
Adjust sequences to enhance protein expression efficiency.
Custom Vectors & Restriction Sites
150+ vector options available; clients may also provide their own.
Synthesis of Challenging DNA
Handles high GC content, repetitive sequences, and hairpin structures.
100% Sequence Accuracy Guarantee
Verified by sequencing + restriction enzyme digestion.
Synthesis Process Description
Upon providing your designed DNA sequence, we can arrange for its construction into the designated vector. If codon optimization analysis is required, please provide the amino acid sequence and expression system information, and we will assist you in achieving your ideal design. You are free to specify restriction enzyme sites, add stop codons, or remove particular enzyme sites, enabling a truly customized molecular cloning project.
1. Submit Sequence and Requirements
Including vector/restriction enzyme/host conditions
2. Analysis and Design
Codon optimization and sequence design
3. Synthesis and Delivery
QC completed, on-time shipment
Areas of Application
Our gene synthesis technology is widely applied across various research and development projects, assisting researchers in overcoming limitations related to sample availability, complex designs, and expression efficiency issues:
Genes difficult to obtain via PCR
Samples difficult to acquire or with confidential origins
Multi-site mutagenesis design
Codon optimization to enhance protein expression
Gene libraries, large cDNA fragment applications
Design and synthesis of DNA vaccines and gene therapy vectors
How to Order Gene Synthesis from Yao-Hong?
Simply email us your designed DNA sequence, and we will arrange for the construction of your designed gene sequence into your desired vector (please inform us in advance if specific enzyme sites are required). Our company offers 100% accurate full gene synthesis services and provides "gene sequence optimization analysis" for your reference.
We can currently successfully synthesize genes up to 50,000 bp. Our extensive synthesis experience means you no longer need to synthesize large fragment primers yourself to piece together your ideal gene.
To perform "gene sequence optimization analysis," please provide the following information so we can offer you recommended designs:
-
Coding region or protein sequence.
-
The expression vector you want to use next? (if the vector is uncommercial or revised by customer, please also provide the map and sequence of the vector)
-
Expression host system?
-
Do you need a stop codon?
-
Do you want to exclude any restriction enzyme site(s) in the optimized gene?
-
Do you need any restriction enzyme site flanking the gene?
Ordering Information
Order Line: +886-2-26686845
E-mail：yaohong@yh-bio.com.tw
Line ID：yaohong26686845