Cat. # AG-40B-0181
Synonyms：V-type Immunoglobulin Domain-containing Suppressor of T Cell Activation; Platelet Receptor Gi24; Stress-induced Secreted Protein-1; Sisp-1; V-set Domain-containing Immunoregulatory Receptor
Source/Host：HEK 293 cells
Sequence：The extracellular domain of mouse VISTA (aa 33-191) is fused at the C-terminus to the domain of the mouse cartilage oligomeric matrix protein (COMP) (aa 28-72) and a His Tag.
MW：~35-40kDa (SDS-PAGE) and >250kDa (Size Exclusion Chromatography (SEC))
Endotoxin Content：<0.01EU/μg purified protein (LAL test; Lonza).
Concentration：1mg/ml after reconstitution.
Reconstitution：Reconstitute with 50μl sterile water.
Formulation：Lyophilized. Contains PBS.
Other Product Data：UniProt link Q9D659: VISTA (mouse)
Shipping and Handling
Short Term Storage：+4°C
Long Term Storage：-20°C
Handling Advice：After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability：Stable for at least 6 months after receipt when stored at -20°C.
VISTA (mouse):COMP (mouse) (rec.) (His)
Mouse VISTA is a 55 to 65kDa type I Ig membrane protein with the extracellular domain homologous to PD-L1. VISTA is mainly expressed on hematopoietic tissues (spleen, thymus and bone marrow) and on myeloid cells with lower expression on CD4+ and CD8+ T cells. VISTA is a new negative checkpoint regulator that potently suppresses T cell activation. Overexpression of VISTA on tumors inhibits the protective anti-tumor immunity and blockade of VISTA enhances antitumor immunity in multiple tumor models. VISTA on APC delivers negative signaling to T cells via a yet-to-identify binding partner on T cells. Recently, it has been reported that recombinant VISTA protein needs to be multimerized to be active as soluble ligand. The protein VISTA (mouse):COMP (mouse) (with the extracellular domain of VISTA (mouse) fused to the pentamerization domain from the cartilage oligomeric matrix protein (COMP)), but not VISTA-Fc, functions as an immunosuppressive agonist in vivo inhibiting the proliferation of CD4+ T cells.
VISTA.COMP - an engineered checkpoint receptor agonist that potently suppresses T cell-mediated immune responses: A. Prodeus, et al.; JCI Insight 2, e94308 (2017)