Cat. # AG-40B-0181

Product Details

Synonyms:V-type Immunoglobulin Domain-containing Suppressor of T Cell Activation; Platelet Receptor Gi24; Stress-induced Secreted Protein-1; Sisp-1; V-set Domain-containing Immunoregulatory Receptor

Product Type:Protein

Properties

Source/Host:HEK 293 cells

Sequence:The extracellular domain of mouse VISTA (aa 33-191) is fused at the C-terminus to the domain of the mouse cartilage oligomeric matrix protein (COMP) (aa 28-72) and a His Tag.

Crossreactivity:Mouse

MW:~35-40kDa (SDS-PAGE) and >250kDa (Size Exclusion Chromatography (SEC))

Purity:≥95% (SDS-PAGE)

Endotoxin Content:<0.01EU/μg purified protein (LAL test; Lonza).

Concentration:1mg/ml after reconstitution.

Reconstitution:Reconstitute with 50μl sterile water.

Formulation:Lyophilized. Contains PBS.

Other Product Data:UniProt link Q9D659: VISTA (mouse)

Shipping and Handling

Shipping:BLUE ICE

Short Term Storage:+4°C

Long Term Storage:-20°C

Handling Advice:After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.PBS containing at least 0.1% BSA should be used for further dilutions.

Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.

VISTA (mouse):COMP (mouse) (rec.) (His)

  • Mouse VISTA is a 55 to 65kDa type I Ig membrane protein with the extracellular domain homologous to PD-L1. VISTA is mainly expressed on hematopoietic tissues (spleen, thymus and bone marrow) and on myeloid cells with lower expression on CD4+ and CD8+ T cells. VISTA is a new negative checkpoint regulator that potently suppresses T cell activation. Overexpression of VISTA on tumors inhibits the protective anti-tumor immunity and blockade of VISTA enhances antitumor immunity in multiple tumor models. VISTA on APC delivers negative signaling to T cells via a yet-to-identify binding partner on T cells. Recently, it has been reported that recombinant VISTA protein needs to be multimerized to be active as soluble ligand. The protein VISTA (mouse):COMP (mouse) (with the extracellular domain of VISTA (mouse) fused to the pentamerization domain from the cartilage oligomeric matrix protein (COMP)), but not VISTA-Fc, functions as an immunosuppressive agonist in vivo inhibiting the proliferation of CD4+ T cells. 

    Product References

    VISTA.COMP - an engineered checkpoint receptor agonist that potently suppresses T cell-mediated immune responses: A. Prodeus, et al.; JCI Insight 2, e94308 (2017)

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