top of page
PD-L1 (human) ELISA Kit   96 wells

Cat. # AG-45B-0016-KI01

 

Product Details
SynonymsProgrammed Cell Death 1 Ligand Protein 1; PDCD1 Ligand 1; B7-H1; PDL1; CD274
Product TypeKit
Properties
Application SetQuantitative ELISA
Specificity

Detects soluble human PD-L1 (sPD-L1) in serum, plasma and cell culture supernatant.

CrossreactivityHuman
Quantity

1 x 96 wells

Sensitivity0.8pg/ml
Range2.34 to 150pg/ml
Sample TypeCell Culture Supernatant
Plasma
Serum
Assay TypeSandwich
Detection TypeColorimetric
Other Product Data

UniProt link Q9NZQ7: PD-L1 (human)

DeclarationIn collaboration with Suzhou Bright Scistar Biotechnology
Accession NumberQ9NZQ7
Shipping and Handling
ShippingBLUE ICE
Short Term Storage+4°C
Long Term Storage+4°C
Handling AdviceAfter standard reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Plate and reagents should reach room temperature before use.
Use/Stability12 months after the day of manufacturing. See expiry date on ELISA Kit box.

PD-L1 (human) ELISA Kit 96 wells

  • PD-L1 (also known as Programmed Cell Death 1 Ligand Protein 1; CD274 or B7-H1) is an immuno-coinhibitory member of the B7 family and functions as an immune checkpoint via its interaction with its receptors PD-1 (CD279) and CD80 (also known as B7-1). PD-L1 is expressed by hematopoietic cells such as T, B and dendritic cells and by parenchymal cells in response to cytokine (i.e. IFN-γ) induction. Ligation of PD-1 by PD-L1 (B7-H1; CD274) or PD-L2 (B7-DC; CD273) inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ and also inhibits BCR mediated signaling. PD-L1 binding to CD80 inhibits T cells proliferation. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Soluble form of PD-L1 (sPD-L1) is expressed upon immune cells activation and can be secreted in the serum/plasma. Soluble checkpoints, such as sPD-1 or sPD-L1, are involved in positive or negative immune regulation and changes in their serum/plasma levels affect the development, prognosis and treatment of cancer. Increase of sPD-L1 or sPD-1 levels have been detected in patients with different types of cancer. This PD-L1 (human) ELISA kit is developed to detect specifically sPD-L1 (human) in different biological fluids.

bottom of page