Cat. # AG-45B-0015-KI01
| Product Details | |
|---|---|
| Synonyms | CD279; Programmed Cell Death Protein 1 |
| Product Type | Kit |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity | Detects soluble human PD-1 (sPD-1) in serum, plasma and cell culture supernatant. |
| Crossreactivity | Human |
| Quantity | 1 x 96 wells |
| Sensitivity | 1.6pg/ml |
| Range | 3.125 to 200pg/ml |
| Sample Type | Cell Culture Supernatant Plasma Serum |
| Assay Type | Sandwich |
| Detection Type | Colorimetric |
| Other Product Data | UniProt link Q15116: PD-1 (human) |
| Declaration | In collaboration with Suzhou Bright Scistar Biotechnology |
| Accession Number | Q15116 |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice | After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use. |
| Use/Stability | 12 months after the day of manufacturing. See expiry date on ELISA Kit box. |
PD-1 (human) ELISA Kit 96 wells
PD-1 (Programmed Cell Death Protein 1; CD279) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses. Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or downregulate T cell activation (CTLA-4 and PD-1). PD-1 is expressed on activated T cells, B cells, myeloid cells and on a subset of thymocytes. Ligation of PD-1 by PD-L1 (B7-H1; CD274) or PD-L2 (B7-DC; CD273) inhibits TCR-mediated T cell proliferation and production of IL-1, IL-4, IL-10 and IFN-γ. In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Soluble form of PD-1 (sPD-1) is expressed upon immune cells activation and can be secreted in the serum/plasma. Soluble checkpoints, such as sPD-1 or sPD-L1, are involved in positive or negative immune regulation and changes in their serum/plasma levels affect the development, prognosis and treatment of cancer. Increase of sPD-1 or sPD-L1 levels have been detected in patients with different types of cancer. This PD-1 (human) ELISA kit is developed to detect specifically sPD-1 (human) in different biological fluids.