＊全球最落實執行驗證制度的抗體公司：依循Nature Methods 。
＊ATLAS antibodies 抗體皆為瑞典原廠獨立研製。
＊人類細胞株、檢體 IHC 最佳的選擇。
＊耗資千萬的增強驗證：1) genetic strategy, 2) orthogonal strategy, 3) independent antibody strategy, 4) expression of tagged proteins strategy and, 5) immunocapture followed by mass spectrometry strategy.
The PrEST antigens are used for immunization. The resulting polyclonal antibodies are affinity purified against the corresponding PrEST Antigens in a three-step procedure to remove the tag-specific antibodies and to catch the PrEST-specific.
PrEST Antigen Design
The 50-150 protein specific fragments are selected using a proprietary software to contain unique epitopes present in the native protein suitable for triggering the generation of antibodies of high specificity.
The high specificity is achieved by a complete human genome scanning to ensure that regions with the lowest homology to other human proteins are used as antigens for the generation of antibodies.
PrEST Antigen Production
For cloning of the PrESTs, a pool of RNA consisting of material from a number of human tissues is used in an RT-PCR approach. The amplified gene fragments are cloned and sequence verified. An E. coli recombinant protein expression system is used for expressing the clones and the products are purified using nickel-containing matrices (IMAC).
PrEST Antigen Validation and Quality Control
The expressed PrEST Antigens are validated using ESI mass spectrometry. Purity is analyzed using SDS page and the PrEST Antigen amount is being quantified using the Nanodrop system.
Triple A Polyclonals：最高階專一性、再現性和多功能性的多株抗體
Atlas Antibodies Advanced Polyclonals (Triple A Polyclonals) are advanced research grade rabbit polyclonal antibodies developed within the Human Protein Atlas project. The antigens are carefully designed, using a proprietary software to select the most suitable antigen for a given target protein. To secure that the very highest levels of specificity, reproducibility and versatility are achieved, a unique antibody purification process using the recombinant antigen as affinity ligand is performed. You can find all Triple A Polyclonals here in our product catalog.
Our catalog includes 21,000 Triple A Polyclonals that target more than 75% of the human protein coding genes.
Developed by top Swedish scientists for the creation of the Human Protein Atlas, our carefully designed Triple A Polyclonals are produced using the same standardized production process to ensure consistency. Our unique antibody purification process, using the recombinant PrEST-antigen as the affinity ligand, then achieves the very highest level of reproducibility New production lots are tested in parallel with existing lots to ensure functionality and assay reproducibility in a real lab situation.
Each Triple A Polyclonal antibody has been used for IHC-staining of samples from 708 individuals originating from normal and cancer afflicted human tissues. Trained pathologists have evaluated the reliability of all IHC-staining patterns. In addition ICC-IF-based confocal microscopy has been used to study subcellular distribution patterns in selected cell-lines. Further, Triple A Polyclonals are characterized by Western Blot (WB), primarily in endogenous protein lysates from human tissues.
Triple A Polyclonals combine optimal specificity with superior versatility across applications including IHC, IF and WB. While anti-peptide antibodies often generate too narrowly specific antibodies, the use of our PrEST-antigens results in advanced polyclonals that combine optimal specificity and minimal off-target binding with superior epitope recognition across applications, protein states and species.
讓Atlas Antibodies陪您盡情探索human proteome
If you would like to use Triple A Polyclonals in an application or species we have not yet evaluated, we encourage you to participate in our Explorer Program.
PrecisA Monoclonals：Precise, Accurate and Targeted Monoclonal Antibodies
PrecisA Monoclonals are our mouse monoclonal antibodies. We are developing monoclonal antibodies against a number of carefully selected targets as a strong complement to our rabbit polyclonal antibody product portfolio. See all PrecisA Monoclonals in our online catalog.
The name PrecisA Monoclonals originates from our core values at Atlas Antibodies and it also reflects our Swedish origin. The Swedish word “precisa” means precise, accurate and targeted. We find this a perfect description for our in-house developed mouse monoclonal antibodies.
Atlas Antibodies take special care in offering clones recognizing only unique non-overlapping epitopes and/or isotypes. By using our stringent production process and characterization procedure, our monoclonal antibodies have a defined specificity, multiplexing opportunities, secured continuity and a stable supply, overall offering premium performance in approved applications. They also permit high working dilutions and contribute to standardized assay procedures.
The Protein Epitope Signature Tag (PrEST) concept gives you antibody performance built in from start. Using proprietary bioinformatics software, 50-150 amino acid regions with the lowest possible sequence identity to other human proteins are selected, cloned and recombinantly produced in a tightly controlled set-up.
Functional characterization is performed on a large number of ELISA positive cell supernatants to select the optimal clones for each application prior to subcloning and expansion of selected Hybridomas.
PrecisA Monoclonals are epitope-mapped using synthetic overlapping peptides in a bead-based array format for selection of clones with non-overlapping epitopes only. Using this method, we can determine the precise binding site of the antibody to its target protein.
All our PrecisA Monoclonal antibodies are isotyped to allow for multiplexing using isotype-specific secondary antibodies.
Hybridoma Cell Cultivation
PrecisA Monoclonals are cultured in murine cell lines.
The characterization of PrecisA Monoclonals starts with an extensive literature search to select the most accurate, relevant and clinically significant tissues to use for immunohistochemistry (IHC) characterization. We often present several tissue types in the IHC application data in our product catalog. In addition to positive stained tissue we also display a negative control tissue staining and if relevant clinical cancer tissue staining
The characterization for Western blot (WB) data follows that same working procedure and starts with a profound literature search to find the best matching lysate.
Each PrecisA Monoclonal antibody is supplied with the most relevant characterization data for its specific target.